|
KMID : 1025520050470050711
|
|
Journal of Animal Science and Technology
2005 Volume.47 No. 5 p.711 ~ p.720
|
|
|
Immunohistochemical Study on the TNFα-Secreting Macrophages and Endothelial Cells in the Porcine Corpus Luteum
|
|
Park Chang-Sik
Han Seung-Ro
Kim Su-Il
Cho Keun-Ja
Kim Won-Sik
|
|
|
Abstract
|
|
|
In the corpus luteum, TNFα is known to induce functional and structural luteolysis. In addition, it acts as luteotropic agent during the initial and early stage of luteal development. In spite of its importance in corpus luteal development, there is still different opinions for the source cells of TNFα in the corpus luteum. One is the macrophages only, and the other is macrophages are the main source and endothelial cells are the minor source.
In this experiment, using the porcine corpora lutea of pregnancy and ovulatory stages, hematoxylin-eosin stain, macrophage and TNFα immunohistochemistry were carried to reveal the sources of TNFα. As a result, MAC 387-positive macrophages were present in all the stages of corpora lutea. In the mature corpora lutea of nonpregnant stages, the sites of MAC 387-positive macrophages and those of TNFα- positive macrophages were coincided, and the sites of endothelial cells and those of TNFα-positive endothelial cells were nearly coincided. But, in the mature CL of pregnant stage, mid- and advanced luteolytic stages of both nonpregnant and pregnant stages, the sites of MAC 387-positive macrophages and those of TNFα-positive macrophages were coincided, but not in the endothelial cells. Accordingly, it can be concluded that macrophages are the main source of TNFα in the corpus luteum and endothelial cells are the minor source in the mature and mid-lytic stages, but, in the advanced luteolytic stage, macrophages are the only source of TNFα.
|
|
|
KEYWORD
|
|
|
Corpus Luteum, Macrophage, Endothelial cell, TNFα, Immunohistochemistry
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|